KMID : 0360220090500101569
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Journal of the Korean Ophthalmological Society 2009 Volume.50 No. 10 p.1569 ~ p.1575
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Effect of Methylglyoxal on the Oxidative Stress in Trabecular Meshwork Cells
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Lee Seung-Hee
Kim Jae-Woo Kim Sin-Hoo
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Abstract
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Purpose: To investigate the effect of methylglyoxal (MG), intermediate metabolite of advanced glycation end products(AGE), on the induction of oxidative stress in human trabecular meshwork cells (HTMC).
Methods: Primarily cultured HTMC were exposed to at concentrations of 0, 30, 100, and 300 ¥ìM of MG for 18 hours, with or without co-exposure to N-acetyl-cysteine. Cellular survival and apoptosis were assessed by MTT assay and flow cytometry using annexin-PI double staining. Production of nitric oxide (NO), superoxide, and reactive oxygen species (ROS) was assessed by Griess assay, cytochrome c assay, and dichlorofluorescein diacetate assay, respectively.
Results: MG did not affect cellular survival at concentrations under 100 ¥ìM, but induced apoptosis of HTMC at concentrations over 100 ¥ìM. MG decreased NO production, accompanied with increased superoxide production. In addition, MG increased ROS, which were abolished by N-acetylcysteine.
Conclusions: MG induced oxidative stress by decreasing NO production, accompanied by increasing superoxide and ROS productions in HTMC. AGE could induce trabecular meshwork dysfunction.
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KEYWORD
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Advanced glycation end products, Methylglyoxal, Oxidative stress, Trabecular meshwork cells
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